Gene silencing analyses against amyloid precursor protein (APP) gene family by RNA interference

Tokiko Sakai; Hirohiko Hohjoh

doi:10.1016/j.cellbi.2006.06.018

Gene silencing analyses against amyloid precursor protein (APP) gene family by RNA interference

Cell Biol Int. 2006 Nov;30(11):952-6. doi: 10.1016/j.cellbi.2006.06.018. Epub 2006 Jul 5.

Authors

Tokiko Sakai¹, Hirohiko Hohjoh

Affiliation

¹ National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

PMID: 16889988
DOI: 10.1016/j.cellbi.2006.06.018

Abstract

Amyloid precursor protein (APP) and amyloid precursor-like proteins 1 and 2 (APLP1 and APLP2) are members of a large gene family. Although APP is known to be the source of the beta-amyloid peptides involved in the development of Alzheimer's disease, the normal functions of APP, APLP1 and APLP2 in cells are poorly understood. In this study, we carried out gene silencing analysis by means of RNA interference with synthetic small interfering RNA duplexes targeting the App, Aplp1 and Aplp2 genes in Neuro2a (N2a) cells, a mouse neuroblastoma cell line. The results demonstrated that cell viability and neurite outgrowth of N2a cells undergoing knockdown of Aplp1 were significantly reduced, compared with N2a cells undergoing knockdown of either App or Aplp2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid beta-Protein Precursor / deficiency*
Amyloid beta-Protein Precursor / genetics*
Amyloid beta-Protein Precursor / metabolism
Animals
Cell Survival
Gene Expression Profiling
Mice
Neuroblastoma / pathology
RNA Interference*
RNA, Small Interfering / metabolism

Substances

Amyloid beta-Protein Precursor
Aplp1 protein, mouse
Aplp2 protein, mouse
RNA, Small Interfering