Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Nov 21;844(1):112-8. doi: 10.1016/j.jchromb.2006.07.012. Epub 2006 Aug 4.


The potential importance of the methylation cycle during embryonic development necessitates the establishment of methodology to detect alterations in the relative abundance of s-adenosylmethionine (SAM) and s-adenosylhomocysteine (SAH) in an embryonic experimental system. We have developed a precise and sensitive method for measurement of SAM and SAH based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in single neurulation-stage mouse embryos. Use of a penta-fluorinated high-performance liquid chromatography (HPLC) stationary phase gave enhanced sensitivity due to optimal ionisation in organic mobile phase and increased retention time compared to standard reversed-phase separation. Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02-25.0microM, SAH 0.01-10.0microM) were linear (r(2)>0.997) with limits of detection for SAM and SAH of 10 and 2.5nmol/L, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Embryo, Mammalian / chemistry*
  • Female
  • Male
  • Mice
  • Pregnancy
  • Reproducibility of Results
  • S-Adenosylhomocysteine / analysis*
  • S-Adenosylmethionine / analysis*
  • Sensitivity and Specificity
  • Tandem Mass Spectrometry / methods*


  • S-Adenosylmethionine
  • S-Adenosylhomocysteine