Mitotic activation of the kinase Aurora-A requires its binding partner Bora

Dev Cell. 2006 Aug;11(2):147-57. doi: 10.1016/j.devcel.2006.06.002.

Abstract

The protein kinase Aurora-A is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis. Here, we describe the identification of Bora, a conserved protein that is required for the activation of Aurora-A at the onset of mitosis. In the Drosophila peripheral nervous system, bora mutants have defects during asymmetric cell division identical to those observed in aurora-A. Furthermore, overexpression of bora can rescue defects caused by mutations in aurora-A. Bora is conserved in vertebrates, and both Drosophila and human Bora can bind to Aurora-A and activate the kinase in vitro. In interphase cells, Bora is a nuclear protein, but upon entry into mitosis, Bora is excluded from the nucleus and translocates into the cytoplasm in a Cdc2-dependent manner. We propose a model in which activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate Aurora-A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aurora Kinases
  • CDC2 Protein Kinase / metabolism
  • Cell Division / physiology
  • Cell Line
  • Cells, Cultured
  • Drosophila
  • Drosophila Proteins / metabolism*
  • Humans
  • In Vitro Techniques
  • Mitosis / physiology*
  • Mutation
  • Protein Binding
  • Protein-Serine-Threonine Kinases / metabolism*

Substances

  • Bora protein, Drosophila
  • Drosophila Proteins
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases
  • CDC2 Protein Kinase