Identification of mobile cholesterol compounds in experimental gliomas by (1)H MRS in vivo: effects of ganciclovir-induced apoptosis on lipids

FEBS Lett. 2006 Aug 21;580(19):4746-50. doi: 10.1016/j.febslet.2006.07.062. Epub 2006 Aug 1.


This letter presents a novel identification and analysis of mobile cholesterol compounds in an experimental glioma model by (1)H MRS in vivo. The cholesterol compounds turned out to comprise as much as 17 mol% of MRS visible total lipids. The results also imply partly associated accumulation of (1)H MRS detectable cholesterol compounds and unsaturated lipids during gene therapy-induced apoptosis, and indicate that the contribution of cholesterol compounds cannot be bypassed in spectral lipid analysis. The introduced (1)H MRS approach facilitates a non-invasive follow-up of mobile cholesterol compounds, paving way for studies of tumour cholesterol metabolism in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Apoptosis / drug effects*
  • Brain Neoplasms / metabolism*
  • Cholesterol / metabolism*
  • Female
  • Ganciclovir / pharmacology*
  • Glioma / metabolism*
  • Humans
  • Magnetic Resonance Spectroscopy*
  • Neoplasm Transplantation
  • Rats


  • Antiviral Agents
  • Cholesterol
  • Ganciclovir