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. 1990;46(6):453-9.
doi: 10.1016/0024-3205(90)90089-a.

Rat Gastric Histamine Release: A Sensitive Gastrin Bioassay

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Rat Gastric Histamine Release: A Sensitive Gastrin Bioassay

A K Sandvik et al. Life Sci. .

Abstract

Acid secretion and histamine release from the totally isolated vascularly perfused rat stomach are sensitive parameters for the biological effects of gastrin. By optimalizing the Ca2+ concentration of the vascular perfusate the sensitivity for stimulation of acid secretion was improved from 65 to 16 pmol/l gastrin 1-17 (G-17). Measurement of venous histamine before and after gastrin in each stomach preparation excluded inter-stomach variability and improved the sensitivity to 2 pmol/l G-17. This is the most sensitive gastrin bioassay reported, with a detection limit comparable to that of radioimmunoassay.

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