Sub-diffraction-limit Imaging by Stochastic Optical Reconstruction Microscopy (STORM)

Nat Methods. 2006 Oct;3(10):793-5. doi: 10.1038/nmeth929. Epub 2006 Aug 9.

Abstract

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA / chemistry
  • Fluorescent Dyes / chemistry*
  • Image Interpretation, Computer-Assisted / methods*
  • Microscopy, Fluorescence / methods
  • Nanotechnology / methods
  • Stochastic Processes

Substances

  • Fluorescent Dyes
  • DNA