Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia

Mol Cell Biochem. 1990 Jan 18;92(1):53-60. doi: 10.1007/BF00220719.


Regeneration of cilia in Tetrahymena cells treated with 1 micrograms/ml of 4',6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells-but not DAPI-treated ones-have just restored their motility, revealed that; - 1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; - 2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; - 3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of alpha- and beta-tubulin; - 4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cilia / drug effects*
  • Cilia / physiology
  • Electrophoresis
  • Indoles / pharmacology*
  • Nucleic Acid Denaturation
  • Poly A / analysis*
  • Protein Biosynthesis
  • RNA / analysis*
  • Regeneration / drug effects
  • Tetrahymena pyriformis / drug effects
  • Tetrahymena pyriformis / genetics*
  • Tubulin / biosynthesis


  • Indoles
  • Tubulin
  • Poly A
  • DAPI
  • RNA