Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1911-1916. doi: 10.1099/ijs.0.64354-0.

Abstract

A novel marine bacterial strain, PV-4(T), isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0-42 degrees C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4(T) was iso-C(15 : 0). Strain PV-4(T) had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4(T) within the genus Shewanella. PV-4(T) exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4(T) showed low mean levels of relatedness to the DNAs of S. aquimarina (50.5 %) and S. marisflavi (8.5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4(T) (=ATCC BAA-1088(T)=DSM 17748(T)).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Composition
  • Culture Media
  • Cyanoacrylates / analysis
  • DNA, Bacterial / chemistry
  • Fatty Acids / analysis
  • Genome, Bacterial
  • Iron
  • Marine Biology*
  • Molecular Sequence Data
  • Pacific Ocean
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Homology, Nucleic Acid
  • Shewanella / chemistry
  • Shewanella / classification*
  • Shewanella / isolation & purification
  • Shewanella / physiology
  • Species Specificity
  • Temperature
  • Ubiquinone / analysis
  • Vitamin K 2 / analogs & derivatives
  • Water Microbiology*

Substances

  • Culture Media
  • Cyanoacrylates
  • DNA, Bacterial
  • Fatty Acids
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Ubiquinone
  • cyacrin
  • vitamin MK 7
  • Iron

Associated data

  • GENBANK/DQ286387