Enhancement of the fungicidal activity of amphotericin B by allicin, an allyl-sulfur compound from garlic, against the yeast Saccharomyces cerevisiae as a model system

Planta Med. 2006 Oct;72(13):1247-50. doi: 10.1055/s-2006-947203. Epub 2006 Aug 10.

Abstract

Amphotericin B (AmB) is a representative antibiotic for the control of serious fungal infections, and its fungicidal activity was greatly enhanced by allicin, an allyl-sulfur compound from garlic. In addition to the plasma membrane permeability change, AmB induced vacuole membrane damage so that the organelles were visible as small discrete particles. Although allicin was ineffective in promoting AmB-induced plasma membrane disability, this compound enhanced AmB-induced structural damage to the vacuolar membrane even at a non-lethal dose of the antibiotic. Allicin could also enhance the antifungal activity of AmB against the pathogenic fungus Candida albicans and against Aspergillus fumigatus. In contrast, allicin did not enhance the cytotoxic activity of AmB against cells of human promyelocytic leukemia (HL-60), a vacuole-less organism.

MeSH terms

  • Amphotericin B / chemistry
  • Amphotericin B / pharmacology*
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Aspergillus fumigatus / drug effects
  • Aspergillus fumigatus / ultrastructure
  • Candida albicans / drug effects
  • Candida albicans / ultrastructure
  • Cell Membrane Permeability / drug effects
  • Disulfides
  • Drug Synergism
  • Garlic / chemistry
  • Intracellular Membranes / drug effects
  • Intracellular Membranes / ultrastructure
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / ultrastructure
  • Sulfinic Acids / chemistry
  • Sulfinic Acids / isolation & purification
  • Sulfinic Acids / pharmacology*
  • Vacuoles / drug effects
  • Vacuoles / ultrastructure

Substances

  • Antifungal Agents
  • Disulfides
  • Sulfinic Acids
  • allicin
  • Amphotericin B