Consumption of saturated fat impairs the anti-inflammatory properties of high-density lipoproteins and endothelial function

J Am Coll Cardiol. 2006 Aug 15;48(4):715-20. doi: 10.1016/j.jacc.2006.04.080. Epub 2006 Jul 24.


Objectives: The purpose of this study was to investigate the influence of dietary fatty acids on the anti-inflammatory properties of high-density lipoproteins (HDL) and vascular function.

Background: The effect of dietary fatty acids on atherogenesis remains uncertain.

Methods: Fourteen adults consumed an isocaloric meal containing either a polyunsaturated or a saturated fat on 2 occasions. The effects of post-prandial HDL on endothelial cell expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were determined. Flow-mediated dilation (FMD) and microvascular reactivity were assessed before and 3 and 6 h after the meal.

Results: Plasma triglycerides, insulin, and nonesterified fatty acids rose after the meals. The HDL collected 6 h after the saturated meal were less effective than HDL isolated from fasting plasma in terms of their ability to inhibit expression of ICAM-1 and VCAM-1, whereas HDL collected 6 h after the polyunsaturated meal had an inhibitory activity that was greater than that of HDL collected from fasting plasma (p < 0.004 and p = 0.01 for comparison of effect of meals on ICAM-1 and VCAM-1, respectively). Post-hyperemic microvascular flow significantly increased at 3 h after the polyunsaturated meal by 45 +/- 14% and by 21 +/- 11% after the saturated meal. The FMD decreased 3 h after the saturated meal by 2.2 +/- 0.9% (p< 0.05 compared with baseline) and by 0.9 +/- 1% after the polyunsaturated meal.

Conclusions: Consumption of a saturated fat reduces the anti-inflammatory potential of HDL and impairs arterial endothelial function. In contrast, the anti-inflammatory activity of HDL improves after consumption of polyunsaturated fat. These findings highlight novel mechanisms by which different dietary fatty acids may influence key atherogenic processes.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers / metabolism
  • Cholesterol, HDL / immunology
  • Cholesterol, HDL / metabolism*
  • Cullin Proteins / metabolism
  • Dietary Fats / adverse effects*
  • Endothelium, Vascular / metabolism
  • Female
  • Hemodynamics
  • Humans
  • Inflammation
  • Intercellular Adhesion Molecule-1 / metabolism
  • Male
  • Middle Aged
  • Postprandial Period
  • Receptors, Vasopressin / metabolism


  • Biomarkers
  • CUL5 protein, human
  • Cholesterol, HDL
  • Cullin Proteins
  • Dietary Fats
  • Receptors, Vasopressin
  • Intercellular Adhesion Molecule-1