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Comparative Study
. 2006 Aug 23;54(17):6159-65.
doi: 10.1021/jf061241e.

Technique for Rapid, Small-Scale Analysis of Vitamin C Levels in Fruit and Application to a Tomato Mutant Collection

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Comparative Study

Technique for Rapid, Small-Scale Analysis of Vitamin C Levels in Fruit and Application to a Tomato Mutant Collection

Rebecca Stevens et al. J Agric Food Chem. .

Abstract

We present a technique for easy, rapid analysis of both total and reduced forms of vitamin C in fruits using microplates and a plate reader. This technique has been compared with a spectrofluorometric technique classically used for assaying vitamin C in fresh tomato. We have applied these methods to a population of 118 tomato mutant lines and controls in search of variability for this trait. Six lines, identified as having high vitamin C levels, and four lines having low vitamin C levels have been chosen for further study. The vitamin C levels have been compared with sugar concentration, dry matter content, fruit weight, titratable acidity, and firmness. The correlations that often exist in tomato varieties between sugar and vitamin C content (positive correlation) or fruit weight and vitamin C content (negative correlation) can be uncoupled in the lines selected for further analysis.

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