Spectroscopic investigation of the function of aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution as a dentin desensitizer

Eur J Oral Sci. 2006 Aug;114(4):354-9. doi: 10.1111/j.1600-0722.2006.00382.x.


Fourier-transform (FT)-Raman and -infrared (IR) spectroscopy were employed to investigate the function of the aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution (Gluma) as a desensitizer. 2-Hydroxyethylmethacrylate (HEMA), glutaraldehyde (GA), and the mixture of HEMA/GA (i.e. Gluma) were used to interact with dentin, collagen, hydroxyapatite (HAP), and bovine serum albumin (BSA) individually. All the interactions were monitored by an FT-Raman spectrometer. FT-IR spectroscopy was also used in this study. The results show that HEMA could be absorbed by dentin and collagen; GA could cross-link collagen and BSA; and when BSA was added to Gluma, polymerization of HEMA occurred. The results suggest that Gluma acts as a desensitizer whereby, first, GA reacts with part of the serum albumin in dentinal fluid, which induces a precipitation of serum albumin, then, second, a reaction of GA with serum albumin induces polymerization of HEMA. The function of Gluma as a desensitizer to block dentinal tubules occurs via these two reactions.

MeSH terms

  • Absorption
  • Animals
  • Cattle
  • Chemical Precipitation
  • Collagen Type I / drug effects
  • Cross-Linking Reagents / chemistry
  • Dentin / drug effects*
  • Dentin Sensitivity / drug therapy*
  • Durapatite / chemistry
  • Glutaral / chemistry
  • Glutaral / therapeutic use*
  • Methacrylates / chemistry
  • Methacrylates / therapeutic use*
  • Polymers / chemistry
  • Serum Albumin / drug effects
  • Spectrophotometry, Infrared
  • Spectroscopy, Fourier Transform Infrared


  • Collagen Type I
  • Cross-Linking Reagents
  • Methacrylates
  • Polymers
  • Serum Albumin
  • gluma desensitizer
  • hydroxyethyl methacrylate
  • Durapatite
  • Glutaral