Identification of derlin-1 as a novel growth factor-responsive endothelial antigen by suppression subtractive hybridization

Biochem Biophys Res Commun. 2006 Oct 6;348(4):1272-8. doi: 10.1016/j.bbrc.2006.07.186. Epub 2006 Aug 7.


Endothelial cells play an important regulatory role in embryonic development, reproductive functions, tumor growth and progression. In the present study, the suppression subtractive hybridization (SSH) method was employed to identify differentially expressed genes between non-stimulated endothelial cells and activated endothelial cells. Following mRNA isolation of non-stimulated and hepatocellular carcinoma homogenate-stimulated cells, cDNAs of both populations were prepared and subtracted by suppressive PCR. Sequencing of the enriched cDNAs identified a couple of genes differentially expressed, including derlin-1. Derlin-1 was significantly up-regulated by tumor homogenates, VEGF, and endothelial growth supplements in a dose-dependent manner. Knock-down of derlin-1 triggered endothelial cell apoptosis, inhibited endothelial cell proliferation, and blocked the formation of a network of tubular-like structures. Our data reveal that derlin-1 is a novel growth factor-responsive endothelial antigen that promotes endothelial cell survival and growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / metabolism
  • Apoptosis
  • Carcinoma, Hepatocellular / blood supply
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Proliferation
  • Cell Survival
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Humans
  • Liver Neoplasms / blood supply
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • Membrane Proteins / antagonists & inhibitors
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Neoplasms / blood supply*
  • Neovascularization, Pathologic*
  • Nucleic Acid Hybridization / methods
  • Up-Regulation
  • Vascular Endothelial Growth Factor A / pharmacology


  • Antigens, Neoplasm
  • DERL1 protein, human
  • Membrane Proteins
  • Vascular Endothelial Growth Factor A