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. 2006 Nov;188(21):7696-9.
doi: 10.1128/JB.00838-06. Epub 2006 Aug 17.

Serine/threonine Protein Kinase SpkA in Synechocystis Sp. Strain PCC 6803 Is a Regulator of Expression of Three Putative pilA Operons, Formation of Thick Pili, and Cell Motility

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Free PMC article

Serine/threonine Protein Kinase SpkA in Synechocystis Sp. Strain PCC 6803 Is a Regulator of Expression of Three Putative pilA Operons, Formation of Thick Pili, and Cell Motility

Vladimir B Panichkin et al. J Bacteriol. .
Free PMC article

Abstract

Previous studies showed that a Ser/Thr protein kinase, SpkA, in Synechocystis sp. strain PCC 6803 is involved in cell motility. The present study, in which DNA microarray analysis and electron microscopy were used, demonstrated that SpkA regulates the expression of putative pilA9-pilA10-pilA11-slr2018, pilA5-pilA6, and pilA1-pilA2 operons and is essential for the formation of thick pili.

Figures

FIG. 1.
FIG. 1.
Northern blot analysis of changes in expression of the pilA1, pilA5, and pilA9 genes due to mutation of the spkA gene. Fragments of the pilA1, pilA5, pilA9, and rnpB genes were used as probes. WT, wild type.
FIG. 2.
FIG. 2.
Mutant spkA::Cmr cells failed to form thick pili. Wild-type (A) and spkA::Cmr (B) cells were processed by negative staining techniques and examined with an electron microscope. In spkA::Cmr cells thick pili were not observed, and the thin pili were more fragmented than those of wild-type cells. White bars = 0.1 μm; black bars = 1 μm.

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