Murine polyomavirus-like particles induce maturation of bone marrow-derived dendritic cells and proliferation of T cells

Med Microbiol Immunol. 2007 Mar;196(1):31-9. doi: 10.1007/s00430-006-0026-x. Epub 2006 Aug 18.

Abstract

We analysed the effects of murine polyomavirus-like particles (PLPs) on bone marrow-derived dendritic cells (BMDCs) and T cells in vitro. BMDCs activated with PLPs up-regulated CD40, CD80, CD86 and major histocompatibility complex (MHC) class II surface markers and produced proinflammatory cytokines. Chimeric PLPs [expressing the ovalbumin (OVA)-peptides OVA(257-264) or OVA(323-339)], but not wildtype PLPs, activated OVA-specific CD8 T cells and OVA-specific CD4 T cells, respectively, indicating both MHC class I and II presentation of the peptides by antigen-presenting cells. Our results suggest that PLPs may be used as vaccine adjuvants priming dendritic cells to induce potent T cell responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic
  • Animals
  • Antigen Presentation
  • Antigens, CD / immunology
  • Cytokines / immunology
  • Cytokines / metabolism
  • Dendritic Cells / immunology*
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class I / metabolism
  • Histocompatibility Antigens Class II / immunology
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred C57BL
  • Ovalbumin
  • Polyomavirus / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Adjuvants, Immunologic
  • Antigens, CD
  • Cytokines
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Ovalbumin