Histone deacetylase inhibitors reverse gene silencing in Friedreich's ataxia

Nat Chem Biol. 2006 Oct;2(10):551-8. doi: 10.1038/nchembio815. Epub 2006 Aug 20.

Abstract

Expansion of GAA x TTC triplets within an intron in FXN (the gene encoding frataxin) leads to transcription silencing, forming the molecular basis for the neurodegenerative disease Friedreich's ataxia. Gene silencing at expanded FXN alleles is accompanied by hypoacetylation of histones H3 and H4 and trimethylation of histone H3 at Lys9, observations that are consistent with a heterochromatin-mediated repression mechanism. We describe the synthesis and characterization of a class of histone deacetylase (HDAC) inhibitors that reverse FXN silencing in primary lymphocytes from individuals with Friedreich's ataxia. We show that these molecules directly affect the histones associated with FXN, increasing acetylation at particular lysine residues on histones H3 and H4 (H3K14, H4K5 and H4K12). This class of HDAC inhibitors may yield therapeutics for Friedreich's ataxia.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Alleles
  • Anilides / chemical synthesis
  • Anilides / chemistry
  • Anilides / pharmacology*
  • Cell Line
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Enzyme Inhibitors / therapeutic use
  • Friedreich Ataxia / drug therapy
  • Friedreich Ataxia / genetics*
  • Gene Expression Regulation / drug effects
  • Gene Silencing / drug effects*
  • HeLa Cells
  • Heterochromatin / drug effects
  • Heterochromatin / genetics
  • Histone Deacetylase Inhibitors*
  • Histones / chemistry
  • Histones / drug effects
  • Histones / metabolism
  • Humans
  • Iron-Binding Proteins / biosynthesis
  • Iron-Binding Proteins / drug effects
  • Molecular Structure
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Transcription, Genetic / drug effects

Substances

  • Anilides
  • Enzyme Inhibitors
  • Heterochromatin
  • Histone Deacetylase Inhibitors
  • Histones
  • Iron-Binding Proteins
  • N1-(2-aminophenyl)-N8-phenyloctanediamide
  • RNA, Messenger
  • frataxin

Associated data

  • PubChem-Substance/14710626
  • PubChem-Substance/14710627
  • PubChem-Substance/14710628
  • PubChem-Substance/14710629
  • PubChem-Substance/14710630
  • PubChem-Substance/14710631
  • PubChem-Substance/14710632
  • PubChem-Substance/14710633
  • PubChem-Substance/14710634
  • PubChem-Substance/14710635
  • PubChem-Substance/14710636
  • PubChem-Substance/14710637
  • PubChem-Substance/14710638
  • PubChem-Substance/14710639
  • PubChem-Substance/14710640
  • PubChem-Substance/14710641
  • PubChem-Substance/14710642
  • PubChem-Substance/14710643
  • PubChem-Substance/14710644
  • PubChem-Substance/14710645
  • PubChem-Substance/14710646
  • PubChem-Substance/14710647
  • PubChem-Substance/14710648
  • PubChem-Substance/14710649
  • PubChem-Substance/14710650
  • PubChem-Substance/14710651
  • PubChem-Substance/14710652
  • PubChem-Substance/14710653
  • PubChem-Substance/14710654
  • PubChem-Substance/14710655
  • PubChem-Substance/14710656
  • PubChem-Substance/14710657
  • PubChem-Substance/14710658
  • PubChem-Substance/14710659
  • PubChem-Substance/14710660