Encapsulation of single enzyme in nanogel with enhanced biocatalytic activity and stability

J Am Chem Soc. 2006 Aug 30;128(34):11008-9. doi: 10.1021/ja064126t.


Single protein encapsulated into nanogels with uniformed size and controllable shell thickness were prepared by surface acryloylation of a protein molecule followed by aqueous in situ polymerization. Compared to its free counterpart, the encapsulated protein exhibits similar biocatalytic behavior and significantly improved stability at high temperature and in the presence of organic solvent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Enzyme Stability
  • Gels*
  • Horseradish Peroxidase / metabolism*
  • Microscopy, Electron, Transmission
  • Nanostructures*


  • Gels
  • Horseradish Peroxidase