Antihyperglycemic effect of polyphenols from Acerola (Malpighia emarginata DC.) fruit

Biosci Biotechnol Biochem. 2006 Aug;70(8):1813-20. doi: 10.1271/bbb.50592.


A crude acerola polyphenol fraction (C-AP) was prepared by subjecting an acerola extract to a C18 cartridge column, and eluting the adsorbed fraction with ethanol containing 10% of acetic acid. C-AP appeared in a previous study to have an inhibitory effect on alpha-glucosidase and particularly on maltase activities. To elucidate the antihyperglycemic effect of C-AP further, we examined the regulation by C-AP of glucose uptake in Caco-2 cell; this resulted in the inhibition of glucose uptake. We next conducted single administration tests of glucose and maltose to ICR mice to investigate whether C-AP really controlled the intestinal glucose absorption in an animal body. The results showed that C-AP significantly suppressed the plasma glucose level after administering both glucose and maltose, suggesting that C-AP had a preventive effect on hyperglycemia in the postprandial state. The mechanism for this effect is considered to have been both suppression of the intestinal glucose transport and the inhibition of alpha-glucosidase. Despite such a preventive effect, the therapeutic effect of C-AP on hyperglycemia appeared to be low from the experiment with KKAy mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / drug effects
  • Caco-2 Cells
  • Cells, Cultured
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / drug therapy*
  • Disease Models, Animal
  • Flavonoids / administration & dosage
  • Flavonoids / chemistry
  • Flavonoids / pharmacology*
  • Fruit / chemistry*
  • Glucose / metabolism
  • Glucose / pharmacokinetics
  • Glucose / pharmacology
  • Glucose Tolerance Test
  • Humans
  • Hypoglycemic Agents / administration & dosage
  • Hypoglycemic Agents / chemistry
  • Hypoglycemic Agents / pharmacology*
  • Intestinal Absorption / drug effects
  • Intestinal Mucosa / metabolism
  • Male
  • Malpighiaceae / chemistry*
  • Maltose / metabolism
  • Maltose / pharmacology
  • Mice
  • Mice, Inbred ICR
  • Mice, Obese
  • Molecular Structure
  • Phenols / administration & dosage
  • Phenols / chemistry
  • Phenols / pharmacology*
  • Polyphenols
  • Stereoisomerism
  • Time Factors


  • Blood Glucose
  • Flavonoids
  • Hypoglycemic Agents
  • Phenols
  • Polyphenols
  • Maltose
  • Glucose