A rapid method for protein extraction from fission yeast

Biosci Biotechnol Biochem. 2006 Aug;70(8):1992-4. doi: 10.1271/bbb.60087.


Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Proteins / chemistry*
  • Proteins / isolation & purification*
  • Schizosaccharomyces / chemistry*
  • Schizosaccharomyces / cytology
  • Sodium Hydroxide / chemistry*
  • Time Factors


  • Proteins
  • Sodium Hydroxide