Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine model

Biosci Biotechnol Biochem. 2006 Aug;70(8):2013-7. doi: 10.1271/bbb.60260.


We have reported the antiallergic activities of the immunostimulatory oligodeoxynucleotide (ODN) BL07S, identified from genomic DNA of Bifidobacterium longum BB536 from in vitro and in vivo studies. The present study evaluated the efficiency of ODN BL07S in preventing allergic responses by oral administration. Oral administration of BL07S suppressed serum ovalbumin (OVA)-specific immunoglobulin (Ig) E levels and improved the OVA-specific IgG2a/IgG1 ratio. ODN BL07S increased Th1 cytokine and decreased Th2 cytokine production in splenocytes. These results suggest that immunostimulatory ODNs are potentially associated with the antiallergic effects of probiotics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Adjuvants, Immunologic / genetics
  • Administration, Oral
  • Animals
  • Bifidobacterium / genetics*
  • Cytokines / biosynthesis
  • Cytokines / drug effects
  • DNA, Bacterial / genetics*
  • Disease Models, Animal
  • Immunoglobulin E / biosynthesis
  • Immunoglobulin E / drug effects
  • In Vitro Techniques
  • Mice
  • Mice, Inbred BALB C
  • Oligodeoxyribonucleotides / administration & dosage*
  • Oligodeoxyribonucleotides / genetics
  • Spleen / cytology
  • Spleen / metabolism
  • Th1 Cells / drug effects*
  • Th1 Cells / metabolism
  • Th2 Cells / drug effects*
  • Th2 Cells / metabolism


  • Adjuvants, Immunologic
  • Cytokines
  • DNA, Bacterial
  • Oligodeoxyribonucleotides
  • Immunoglobulin E