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, 8 (4), 357-60

Stability of RNA Isolated From Macrophages Depends on the Removal of an RNA-degrading Activity Early in the Extraction Procedure

Affiliations
  • PMID: 1692714

Stability of RNA Isolated From Macrophages Depends on the Removal of an RNA-degrading Activity Early in the Extraction Procedure

R Evans et al. Biotechniques.

Erratum in

  • Biotechniques 1990 Jul;9(1):55

Abstract

Ubiquitous RNases are the usual causes of RNA degradation on its isolation from mammalian cells. Using guanidine hydrochloride for the extraction of RNA from mouse peritoneal macrophages, we identify a major source of RNA-degrading activity, the stage of the extraction procedure at which this activity may be detected and show that its removal early in the extraction leads to a more dependable method for the recovery of high quality RNA.

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