Simplified northern blot hybridization using 5% sodium dodecyl sulfate

Biotechniques. 1990 Apr;8(4):370-1.


We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.

MeSH terms

  • Animals
  • Blotting, Northern / methods
  • DNA Probes
  • Mice
  • Nucleic Acid Hybridization
  • RNA / analysis
  • Sodium Dodecyl Sulfate*


  • DNA Probes
  • Sodium Dodecyl Sulfate
  • RNA