Structural characterization of natural and recombinant human granulocyte colony-stimulating factors

J Biochem. 1990 Mar;107(3):486-92. doi: 10.1093/oxfordjournals.jbchem.a123072.


Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein which stimulates predominantly neutrophilic granulocyte colony formation in mammals. Natural human G-CSF (hG-CSF) and recombinant hG-CSF produced in Chinese hamster ovary (CHO) cells transfected with the cDNA clone for hG-CSF have been purified to apparent homogeneity for structural and biological comparison. The amino acid sequence of recombinant hG-CSF, composed of 174 amino acid residues, was identical with that of natural hG-CSF and also with the sequence predicted from the cDNA. Both forms of hG-CSF have a free Cys-17 and two intramolecular disulfide linkages, between Cys-36 and Cys-42, and between Cys-64 and Cys-74. The O-glycosylation occurred at Thr-133 in both hG-CSFs. Similar CD spectra were obtained for both hG-CSFs. Additionally, both forms showed almost the same biological activities determined by in vitro colony-forming assay and in vivo assay. It is thus concluded that the recombinant hG-CSF is indistinguishable from its natural counterpart and that the former is valuable for more detailed characterization and clinical use.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Cells, Cultured
  • Circular Dichroism
  • Colony-Forming Units Assay
  • Colony-Stimulating Factors / analysis*
  • Colony-Stimulating Factors / pharmacology
  • Cricetinae
  • Cricetulus
  • Disulfides / analysis
  • Granulocyte Colony-Stimulating Factor
  • Humans
  • Hydrolysis
  • Molecular Sequence Data
  • Recombinant Proteins / analysis
  • Recombinant Proteins / pharmacology
  • Serine Endopeptidases


  • Amino Acids
  • Colony-Stimulating Factors
  • Disulfides
  • Recombinant Proteins
  • Granulocyte Colony-Stimulating Factor
  • Serine Endopeptidases
  • glutamyl endopeptidase