Force development at elevated [Mg2+]o and [K+]o in myocardium from the freshwater turtle (Trachemys scripta) and influence of factors associated with hibernation

Comp Biochem Physiol A Mol Integr Physiol. 2006 Nov;145(3):334-9. doi: 10.1016/j.cbpa.2006.07.003. Epub 2006 Jul 13.

Abstract

The effects of high [Mg(2+)](o) on force development were examined for heart muscle of freshwater turtle. Plasma [Mg(2+)] during hibernation may increase drastically and like plasma [K(+)] approach values as high as 10 mM. Each experiment performed at either 20 or 5 degrees C involved four ventricular preparations of which one pair was exposed to 10, and one to 1 mMMg(2+). One preparation of each pair was furthermore exposed to 10 mM K(+), whereas the other was maintained at 2.5 mM K(+). During oxygenation, high relative to low [Mg(2+)](o) displayed a weak tendency to reduce twitch force; a tendency that was not reduced by elevations of [Ca(2+)](o). Severe hypoxia accentuated the negative effect of high [Mg(2+)](o). This effect disappeared, however, when hypoxia was combined with acidosis obtained by 24 mM lactic acid. In comparison to [Mg(2+)](o), high [K(+)](o) strongly depressed force development under both oxygenation and hypoxia, but no consistent interplay between the two ions was revealed. The negative inotropic effects of both high [Mg(2+)](o) and high [K(+)](o) were reduced or eliminated by 10 muM adrenaline. In conclusion the cardiac effects of elevations in [Mg(2+)](o) appear to be small during hibernation, in particular when considering the concomitant adrenergic stimulation and acidosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acidosis / metabolism
  • Animals
  • Calcium / pharmacology
  • Epinephrine / pharmacology
  • Heart / drug effects
  • Heart / physiology*
  • Hibernation / physiology*
  • Hypoxia / metabolism
  • In Vitro Techniques
  • Lactic Acid / pharmacology
  • Magnesium / pharmacology*
  • Myocardial Contraction / drug effects
  • Potassium / pharmacology*
  • Temperature
  • Turtles / physiology*

Substances

  • Lactic Acid
  • Magnesium
  • Potassium
  • Calcium
  • Epinephrine