Monoclonal antibodies block murine IL-4 receptor function

J Immunol. 1990 Jun 1;144(11):4212-7.

Abstract

IL-4 is a cytokine which can induce B-lymphocyte proliferation, increase cell-surface Ia expression, and induce some activated B cells to differentiate and begin to secrete IgE. IL-4 binds specifically to a cell-surface receptor (IL-4R) on cells from a variety of lineages including T and B cells. In general both primary cells and in vitro cell lines express less than 5000 receptors per cell. Utilizing a subclone of the cytotoxic T cell line CTLL-2 expressing a high level of IL-4R, mAb against the murine IL-4R were prepared. Two mAb have been identified which have different properties. These antibodies, designated M1 and M2, recognize sequences specific to the murine IL-4R. Immunoprecipitation studies with M1 and M2 on CTLL-2 cells have identified the receptor as a Mr = 145,000 cell-surface protein. Similar results have been obtained with the recently isolated full length murine IL-4R cDNA expressed in COS-7 cells. In addition the antibodies are capable of inhibiting IL-4 binding. One antibody, M1, is also a potent inhibitor of IL-4-induced proliferation. These antibodies will be useful in dissecting a wide array of activities attributed to IL-4.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Affinity
  • Antibody Specificity
  • Cells, Cultured
  • Epitopes
  • In Vitro Techniques
  • Interleukin-4 / physiology*
  • Lymphocyte Activation
  • Mice
  • Precipitin Tests
  • Receptors, Interleukin-4
  • Receptors, Mitogen / immunology*
  • Receptors, Mitogen / metabolism
  • T-Lymphocytes, Cytotoxic / immunology

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • Interleukin-4