Epidermal transient down-regulation of retinol-binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development

Dev Dyn. 2006 Nov;235(11):3071-9. doi: 10.1002/dvdy.20921.

Abstract

Very little is known about the molecular control of skin patterning and scale morphogenesis in teleost fish. We have found radially symmetrical epidermal placodes with down-regulation of retinol-binding protein 4 (rbp4) expression during the initial paired fin and scale morphogenesis in zebrafish (Danio rerio). This finding may be related to changes in keratinocyte cytodifferentiation and/or the integument retinoid metabolism. rbp4 transcripts are expressed afterward in the central epidermis of the scale papilla and gradually extend to the epidermis, covering the growing scale, whereas no transcripts were detected in posterior margin epidermis. In contrast, induction of apolipoprotein Eb (apoeb) and up-regulation of estrogen receptor 2a (esr2a) transcripts were observed in the epidermis at initiator sites of zebrafish ectodermal/dermal appendage morphogenesis. This expression was maintained in the posterior margin epidermis of the formed scales. esr2a was also strongly expressed in neuromasts, whereas no rbp4 and apoeb transcripts were detected in these mechanosensory structures. The observed epidermal molecular events suggest that epidermis patterning is due to an activator-inhibitor mechanism operational at epidermal-dermal interaction sites. rbp4 transcript expression was also strongly down-regulated by 1-phenyl-2-thio-urea (PTU). As this inhibitor is commonly used to block obscuring pigmentation during in situ hybridization studies, this finding suggests that PTU should be used with caution, particularly in studying skin development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apolipoproteins E / analysis
  • Apolipoproteins E / genetics
  • Carrier Proteins / analysis
  • Carrier Proteins / genetics
  • Down-Regulation
  • Epidermis / chemistry
  • Epidermis / drug effects
  • Epidermis / growth & development*
  • Estrogen Receptor beta
  • Gene Expression Regulation, Developmental*
  • Larva / growth & development
  • Larva / metabolism
  • Morphogenesis / drug effects
  • Morphogenesis / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Receptors, Estrogen / analysis
  • Receptors, Estrogen / genetics
  • Retinol-Binding Proteins, Plasma
  • Thiourea / analogs & derivatives
  • Thiourea / pharmacology
  • Transcription, Genetic / drug effects
  • Up-Regulation
  • Zebrafish / genetics
  • Zebrafish / growth & development*
  • Zebrafish Proteins / analysis
  • Zebrafish Proteins / genetics

Substances

  • Apolipoproteins E
  • Carrier Proteins
  • Estrogen Receptor beta
  • RNA, Messenger
  • Rbp4 protein, zebrafish
  • Receptors, Estrogen
  • Retinol-Binding Proteins, Plasma
  • Zebrafish Proteins
  • apolipoprotein Eb, zebrafish
  • estrogen receptor 2a, zebrafish
  • diphenylthiourea
  • Thiourea