Base sequence specificity of a monoclonal antibody binding to (6-4)photoproducts

Mutat Res. 1990 May;235(3):187-94. doi: 10.1016/0921-8777(90)90073-e.


The DNA base sequence specificity of the 64M-1 monoclonal antibody, which recognizes ultraviolet (UV)-induced (6-4)photoproducts, was characterized. The 64M-1 antibody strongly bound to UV-poly(dU) as well as to UV-poly(dT), and weakly to UV-poly(dC), UV-poly(me5dC) and UV-poly(rU). A competitive inhibition assay using UV-oligo(dT)8, UV-oligo(dTdC)4, UV-oligo(dC)8, UV-PvuI linker (GCGATCGC) and UV-PvuII linker (GCAGCTGC) indicated that the main (6-4)photoproducts detected by the 64M-1 antibody in UV-irradiated DNA are TT(6-4)photoproducts and TC(6-4)photoproducts. Comparison between dTpdT(6-4)photoproduct and dTpdC(6-4)photoproduct showed that the affinity of the 64M-1 antibody for dTpdT(6-4)photoproduct was about 5 times higher than that for dTpdC(6-4)photoproduct. The antibody also binds to isolated TT(6-4)photoproducts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • DNA / immunology*
  • DNA / radiation effects
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Oligonucleotides / radiation effects*
  • Polynucleotides / radiation effects*
  • Pyrimidine Dimers / immunology*
  • Sensitivity and Specificity
  • Tumor Cells, Cultured
  • Ultraviolet Rays


  • Antibodies, Monoclonal
  • Epitopes
  • Oligonucleotides
  • Polynucleotides
  • Pyrimidine Dimers
  • DNA