Interaction of glucagon-like peptide-1 (7-36)amide and cholecystokinin-8 in the endocrine and exocrine rat pancreas

Pancreas. 1990 May;5(3):361-5. doi: 10.1097/00006676-199005000-00019.


The synergistic impact of glucagon-like peptide-1 (GLP-1) (7-36)amide and cholecystokinin-8 (CCK-8) was studied in the rat pancreas. The GLP-1 (7-36)amide (1 pM-1 microM) had no effect on the basal or CCK-stimulated (1 nM-1 pM) amylase release from isolated pancreatic acini. The insulinotropic action of 0.5 nM GLP-1 (7-36)amide, which weakly stimulated the glucose-induced (6.7 mM) insulin release from the isolated perfused rat pancreas, was strongly potentiated by the addition of CCK-8 (20, 50, and 100 pM) to the perfusate. In concentrations as they occur physiologically after a meal, CCK-8 alone had no significant effect on basal or glucose-stimulated (6.7 mM) insulin secretion. Our data support the assumption that the nutrient-regulated intestinal release of various peptides represents a regulatory system to ensure an adequate insulin response to food intake, at least in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amylases / metabolism
  • Animals
  • Drug Synergism
  • Glucagon
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptides
  • In Vitro Techniques
  • Islets of Langerhans / drug effects*
  • Male
  • Pancreas / drug effects*
  • Peptide Fragments*
  • Peptides / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Sincalide / pharmacology*


  • Peptide Fragments
  • Peptides
  • glucagon-like peptide 1 (7-36)amide
  • Glucagon-Like Peptides
  • Glucagon-Like Peptide 1
  • Glucagon
  • Amylases
  • Sincalide