The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding

Cell. 1990 May 18;61(4):611-22. doi: 10.1016/0092-8674(90)90473-r.


The lymph node homing receptor core polypeptide (mLHRc) is composed of a tandem collection of domains: a lectin domain, an epidermal growth factor (EGF) domain, and two repeats common in complement regulatory proteins. Here we demonstrate localization of mLHRc to chromosome 1, the portion syntenic with chromosome 1 in man. This locus is inseparable in mouse strains from the murine lymphocyte cell surface marker Ly-22. The data indicate that Ly-22 is an allelic determinant on the LHR resulting from a single amino acid interchange within the EGF domain. Cross-blocking experiments demonstrate that anti-Ly-22 and MEL-14 recognize independent epitopes and that Ly-22 is distinct from the carbohydrate binding region. Application of anti-Ly-22 in the in vitro binding assay shows inhibition of binding of lymphocytes to high endothelial venules (HEVs). The localization of the Ly-22 epitope in this novel chimeric protein suggests direct participation of the EGF domain in the adhesion of lymphocytes to HEV.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Ly / genetics*
  • Antigens, Surface / genetics
  • Base Sequence
  • Cell Adhesion Molecules / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1
  • Endothelium, Vascular / immunology*
  • Epidermal Growth Factor / genetics
  • Epitopes / analysis
  • Gene Expression
  • Humans
  • Lymph Nodes / immunology*
  • Mice
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • Precipitin Tests
  • Receptors, Immunologic / genetics*
  • Receptors, Lymphocyte Homing
  • Transfection


  • Antigens, Ly
  • Antigens, Surface
  • Cell Adhesion Molecules
  • Epitopes
  • Receptors, Immunologic
  • Receptors, Lymphocyte Homing
  • Epidermal Growth Factor

Associated data

  • GENBANK/M36005
  • GENBANK/M36058