Abstract
Spi-1 is a putative proto-oncogene involved in murine virus-induced acute erythroleukemias. We report here the identification of the human homologue of Spi-1 and its expression in normal and tumorigenic human tissues. Characterization of cDNA clones revealed that the human Spi-1 gene encodes a 216 amino acids protein showing 85% identity with the murine counterpart. By sequencing genomic clones, five exons were identified. To investigate the possible role of Spi-1 gene in human cancers, we studied its expression in a panel of human tumors by Northern blot analysis. Spi-1 expression was detected in all the tumors examined. There was no noticeable evidence of messenger RNA alteration as compared to normal tissues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics*
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Adenocarcinoma / metabolism
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Adenocarcinoma / physiopathology
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Amino Acid Sequence
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Base Sequence*
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Blotting, Northern
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Chromosome Mapping
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Chromosomes, Human, Pair 11 / analysis
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Cloning, Molecular
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DNA Probes
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DNA, Neoplasm / analysis
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Exons
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Humans
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Introns
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Leukemia, Erythroblastic, Acute / genetics*
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Leukemia, Erythroblastic, Acute / metabolism
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Leukemia, Erythroblastic, Acute / physiopathology
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Leukemia, Promyelocytic, Acute / genetics*
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Leukemia, Promyelocytic, Acute / metabolism
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Leukemia, Promyelocytic, Acute / physiopathology
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Lymphoma, Non-Hodgkin / genetics*
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Lymphoma, Non-Hodgkin / metabolism
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Lymphoma, Non-Hodgkin / physiopathology
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Promoter Regions, Genetic
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins / physiology
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RNA / genetics
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Sequence Homology, Nucleic Acid*
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Trans-Activators*
Substances
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DNA Probes
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DNA, Neoplasm
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Trans-Activators
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proto-oncogene protein Spi-1
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RNA