Detection of bacteria by surface-enhanced Raman spectroscopy

Anal Bioanal Chem. 2006 Nov;386(5):1379-86. doi: 10.1007/s00216-006-0711-z. Epub 2006 Aug 25.


The detection and identification of dilute bacterial samples by surface-enhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles. An estimate of the detection limit of E. coli was obtained by varying the concentration of bacteria. By correcting the Raman spectra for the broad librational OH band of water, reproducible spectra were obtained for E. coli concentrations as low as approximately 10(3) cfu/mL. To aid in the assignment of Raman bands, spectra for E. coli in D(2)O are also reported.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Colloids / chemistry
  • Escherichia coli / chemistry*
  • Escherichia coli / growth & development
  • Pseudomonas aeruginosa / chemistry*
  • Pseudomonas aeruginosa / growth & development
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Silver / chemistry
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods*
  • Surface Properties


  • Colloids
  • Silver