The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood

Cell Immunol. 2006 May;241(1):32-7. doi: 10.1016/j.cellimm.2006.07.008. Epub 2006 Aug 28.

Abstract

This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-) cells as a target. We found that Hyper-IL-6 promoted the generation of CD15(+) granulocytic and CD14(+) monocytic cells and suppressed that of CD14(-)CD1a(+) dendritic cells from CD36(-)CD15(-)CD14(-)CD1a(-)IL-6R(+) myeloid progenitors. Conversely, CD34(+)CD38(-) cell-derived early erythroid progenitors were negative for IL-6R expression. Hyper-IL-6 potentiated the generation of CD36(+)glycophorinA(high) mature erythroid cells from the IL-6R(-) early erythroid progenitors. Our results indicate that Hyper-IL-6 augments the generation of CD15(+) granulocytic, CD14(+) monocytic and CD36(+)glycophorinA(high) cell and suppresses that of CD14(-)CD1a(+) dendritic cells.

Publication types

  • Comparative Study

MeSH terms

  • Adjuvants, Immunologic / genetics
  • Adjuvants, Immunologic / physiology*
  • Antigens, CD1 / metabolism
  • Cell Differentiation / immunology*
  • Cells, Cultured
  • Erythroid Precursor Cells / cytology*
  • Erythroid Precursor Cells / immunology
  • Fetal Blood / cytology
  • Fetal Blood / immunology
  • Growth Inhibitors / genetics
  • Growth Inhibitors / physiology
  • Hematopoiesis / genetics
  • Hematopoiesis / immunology
  • Humans
  • Interleukin-6 / genetics
  • Interleukin-6 / physiology*
  • Lewis X Antigen / biosynthesis
  • Lipopolysaccharide Receptors / biosynthesis
  • Lipopolysaccharide Receptors / metabolism
  • Myeloid Progenitor Cells / cytology*
  • Myeloid Progenitor Cells / immunology
  • Receptors, Interleukin-6 / genetics
  • Receptors, Interleukin-6 / physiology*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / physiology*

Substances

  • Adjuvants, Immunologic
  • Antigens, CD1
  • CD1a antigen
  • Growth Inhibitors
  • IL6 protein, human
  • Interleukin-6
  • Lewis X Antigen
  • Lipopolysaccharide Receptors
  • Receptors, Interleukin-6
  • Recombinant Fusion Proteins