Escherichia coli type I isopentenyl diphosphate isomerase: structural and catalytic roles for divalent metals

J Am Chem Soc. 2006 Sep 6;128(35):11545-50. doi: 10.1021/ja063073c.


Isopentenyl diphosphate isomerase (IDI) catalyzes the essential conversion of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP) in the mevalonate entry into the isoprenoid biosynthetic pathway. Two convergently evolved forms of IDI are known. Type I IDI, which is found in Eukarya and many Bacteria, catalyzes the isomerization of IPP and DMAPP by a protonation-deprotonation mechanism. The enzyme requires two divalent metal ions for activity. An X-ray structure of type I IDI from crystals soaked with (N,N-dimethylamino)-1-ethyl diphosphate (NIPP), a potent transition-state analogue for the carbocationic intermediate in the isomerization reaction, shows one of the metals in a His(3)Glu(2) hexacoordinate binding site, while the other forms a bridge between the diphosphate moiety of the substrate and the enzyme (Wouters, J.; et al. J. Biol. Chem. 2003, 278, 11903). Reconstitution of metal-free recombinant Escherichia coli type I IDI with several divalent metals-Mg(2+), Mn(2+), Zn(2+), Co(2+), Ni(2+), and Cd(2+)-generated active enzyme. Freshly purified IDI contained substoichiometric levels of a single metal ion, presumably bound in the hexacoordinate site. When NIPP was added to the disruption and purification buffers of enzyme, the purified protein contained 0.72 equiv of Mg(2+), 0.92 equiv of Zn(2+), and 0.10 equiv of Mn(2+). These results are consistent with a structure in which Mg(2+) facilitates diphosphate binding and Zn(2+) or Mn(2+) occupies the hexacoordinate site.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Buffers
  • Carbon-Carbon Double Bond Isomerases / antagonists & inhibitors
  • Carbon-Carbon Double Bond Isomerases / chemistry*
  • Carbon-Carbon Double Bond Isomerases / metabolism*
  • Catalysis
  • Cations, Divalent
  • Crystallography, X-Ray
  • Escherichia coli / enzymology*
  • Hemiterpenes
  • Magnesium / chemistry
  • Manganese / chemistry
  • Metals / chemistry*
  • Models, Molecular
  • Protein Conformation


  • Buffers
  • Cations, Divalent
  • Hemiterpenes
  • Metals
  • Manganese
  • Carbon-Carbon Double Bond Isomerases
  • isopentenyldiphosphate delta-isomerase
  • Magnesium