Abstract
Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C.
MeSH terms
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Bacillus subtilis / genetics
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Bacillus subtilis / growth & development*
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Bacterial Proteins / genetics
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Biofilms / growth & development*
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Operon / genetics
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Organisms, Genetically Modified / genetics
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Organisms, Genetically Modified / growth & development
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Peptides, Cyclic / biosynthesis*
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Peptides, Cyclic / genetics
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Trans-Activators / genetics
Substances
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Bacterial Proteins
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DegQ protein, Bacillus sp.
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Peptides, Cyclic
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Trans-Activators
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iturin A