Proteomic analysis of root meristems and the effects of acetohydroxyacid synthase-inhibiting herbicides in the root of Medicago truncatula

J Proteome Res. 2006 Sep;5(9):2309-16. doi: 10.1021/pr0600677.


Quantitative proteome analyses of meristematic and nonmeristematic tissues from Medicago truncatula primary and lateral roots and meristem tissues from plants treated with acetohydroxyacid synthase-inhibiting herbicides were made. The accumulation of 81 protein spots changed in meristematic and nonmeristematic tissues and 51 protein spots showed significant changes in accumulation in herbicide-treated meristems. Identified proteins indicate two trends, (i) increased accumulation of cell division and redox-mediating proteins in meristems compared to nonmeristematic tissues and (ii) increased accumulation of pathogenesis-related and decreased accumulation of metabolic proteins in herbicide-treated roots.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetolactate Synthase / antagonists & inhibitors
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation, Plant / drug effects*
  • Herbicides / toxicity*
  • Mass Spectrometry
  • Medicago truncatula / genetics*
  • Meristem / drug effects
  • Meristem / genetics
  • Meristem / metabolism*
  • Plant Proteins / analysis*
  • Proteomics / methods


  • Herbicides
  • Plant Proteins
  • Acetolactate Synthase