Neuro-endocrine interaction on lymphocytes. Testosterone-induced modulation of the lymphocyte substance P receptor

J Neuroimmunol. 1990 Jul;28(2):185-8. doi: 10.1016/0165-5728(90)90033-j.


Human IM-9 B-lymphoblasts have been shown to express the receptor for the neuropeptide substance P (SP). The present study was undertaken to evaluate the effect of sex hormones on this receptor. Testosterone inhibited [125I]SP binding in a dose-dependent manner with an IC50 of approximately 100 nM, while both estradiol and progesterone failed to inhibit SP binding even at concentrations as high as 1 microM. Furthermore, Scatchard analysis indicated that the dissociation constant (Kd) of the SP receptor was markedly increased from 0.25 nM to 2.2 nM when cells were incubated with testosterone. These data indicate a unique neuro-endocrine interaction on lymphocytes involving the substance P receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Dose-Response Relationship, Drug
  • Humans
  • Lymphocytes / metabolism
  • Lymphocytes / physiology*
  • Neurosecretory Systems / physiology*
  • Osmolar Concentration
  • Receptors, Neurokinin-1
  • Receptors, Neurotransmitter / metabolism*
  • Substance P / metabolism
  • Testosterone / pharmacology*


  • Receptors, Neurokinin-1
  • Receptors, Neurotransmitter
  • Substance P
  • Testosterone