Vitronectin and type-I collagen binding by Staphylococcus aureus and coagulase-negative staphylococci

FEMS Microbiol Immunol. 1990 May;2(1):55-62. doi: 10.1111/j.1574-6968.1990.tb03479.x.


Binding of 125I-labelled type-I collagen and 125I-labelled vitronectin (human serum spreading factor or S-protein) was studied using Staphylococcus aureus and coagulase-negative staphylococci of different species. Binding of collagen and vitronectin was time dependent for S. aureus ISP 546, and S. haemolyticus E 2498/86. Co-operative binding of vitronectin and collagen by staphylococcal cells was demonstrated. Binding to S. haemolyticus E 2498/86 was more rapid and was enhanced in vitronectin/collagen mixtures than for either protein separately. Furthermore, pre-incubation of staphylococcal cells with unlabelled collagen enhanced vitronectin binding. When cells of S. haemolyticus E 2498/86 were treated with pronase E, proteinase K, subtilopeptidase A or trypsin, vitronectin-binding was decreased by 50% or more, whereas collagen-binding was protease resistant. For the strains of S. aureus tested, both vitronectin and collagen binding were found to be protease sensitive. Type-I collagen peptides inhibited collagen-binding to S. haemolyticus E 2498/86, whereas vitronectin-binding was not affected perhaps indicating different receptors for these proteins. The binding of both collagen and vitronectin was shown to be reversible, since bound 125I-collagen and 125I-vitronectin were displaced after adding excess of the homologous protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding, Competitive
  • Blood Proteins / metabolism
  • Coagulase / metabolism
  • Collagen / metabolism*
  • Glycoproteins / metabolism*
  • Hot Temperature
  • Humans
  • Kinetics
  • Peptide Hydrolases / metabolism
  • Protein Binding
  • Staphylococcus / growth & development
  • Staphylococcus / metabolism*
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*
  • Vitronectin


  • Blood Proteins
  • Coagulase
  • Glycoproteins
  • Vitronectin
  • Collagen
  • Peptide Hydrolases