The nucleotide sequences of 16S ribosomal-RNA of Streptococcus uberis types I and II were determined by reverse transcriptase. Comparative analysis of the sequence data showed that the two types are phylogenetically distinct and worthy of separate species status. A new species Streptococcus parauberis is proposed for the type II strains. The type strain of Strep. parauberis is NCDO 2020.