Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product

Appl Environ Microbiol. 2006 Sep;72(9):6399-401. doi: 10.1128/AEM.00935-06.

Abstract

The beta-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase family.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genes, Bacterial*
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / isolation & purification
  • Glycoside Hydrolases / metabolism
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Oligosaccharides / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Seawater / microbiology
  • Substrate Specificity
  • Vibrio / enzymology*
  • Vibrio / genetics*
  • Vibrio / isolation & purification

Substances

  • DNA, Bacterial
  • Oligosaccharides
  • Recombinant Proteins
  • Glycoside Hydrolases
  • agarase

Associated data

  • GENBANK/AB218419