Posttranscriptional regulation by the upstream open reading frame of the phosphoethanolamine N-methyltransferase gene

Biosci Biotechnol Biochem. 2006 Sep;70(9):2330-4. doi: 10.1271/bbb.60309. Epub 2006 Sep 7.

Abstract

Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5' untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5' UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the beta-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream beta-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / metabolism
  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Choline / metabolism
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • Gene Expression Regulation, Plant / physiology*
  • Methyltransferases / genetics*
  • Methyltransferases / metabolism
  • Molecular Sequence Data
  • Mutant Chimeric Proteins / genetics
  • Mutant Chimeric Proteins / metabolism
  • Nucleic Acid Hybridization
  • Open Reading Frames*
  • Point Mutation
  • Transcription, Genetic

Substances

  • 5' Untranslated Regions
  • DNA, Plant
  • Mutant Chimeric Proteins
  • Methyltransferases
  • phosphoethanolamine methyltransferase
  • Choline