Rab14 is part of the early endosomal clathrin-coated TGN microdomain

FEBS Lett. 2006 Oct 2;580(22):5241-6. doi: 10.1016/j.febslet.2006.08.053. Epub 2006 Sep 5.

Abstract

Rab14 localizes to the Golgi/TGN and to early endosomes, but its biological function remains unclear. By structural modeling, we identified Rab14-specific residues and established a close relationship between the Rab2/Rab4/Rab14, Rab11/25 and Rab39 sub-groups within the Rab protein family. By quantitative confocal microscopy and by density centrifugation we show that Rab14 is part of the early endosomal AP-1 microdomain. Overexpression of a dominant-negative Rab14 GTP-binding mutant that solely localizes to the Golgi donor compartment accelerated EGF degradation. We suggest that the AP-1 microdomain represents the interconnecting compartment in which Rab14 vesicles cycle between early endosomes and the Golgi cisternae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clathrin / genetics
  • Clathrin / metabolism
  • Clathrin-Coated Vesicles / genetics
  • Clathrin-Coated Vesicles / metabolism*
  • Endosomes / genetics
  • Endosomes / metabolism*
  • Gene Expression
  • Genes, Dominant
  • HeLa Cells
  • Humans
  • Membrane Microdomains / genetics
  • Membrane Microdomains / metabolism*
  • Microscopy, Confocal / methods
  • Mutation
  • Protein Structure, Tertiary / genetics
  • Protein Transport / genetics
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism*
  • trans-Golgi Network / genetics
  • trans-Golgi Network / metabolism*

Substances

  • Clathrin
  • rab GTP-Binding Proteins