No Association Between XRCC1 and XRCC3 Gene Polymorphisms and Breast Cancer Risk: Iowa Women's Health Study

Cancer Detect Prev. 2006;30(4):313-21. doi: 10.1016/j.cdp.2006.07.002. Epub 2006 Sep 11.


Background: Genetic variation in DNA repair may contribute to differences in the susceptibility of several cancers. We evaluated two polymorphisms in the base excision repair pathway (BER) (XRCC1; Arg194Trp and Arg399Gln) and one polymorphism in the double strand DNA repair pathway (XRCC3; Thr241Met) for their association with breast cancer risk.

Methods: The association was analyzed in a nested case control study of 460 breast cancer cases and 324 cancer-free controls within the Iowa Women's Health Cohort. DNA was obtained from blood samples or paraffin embedded tissues (PET) and all samples were genotyped by one of three genotyping platforms-PCR-RFLP, PCR-INVADER, or Sequenom.

Results: None of the three polymorphisms studied were significantly associated with breast cancer risk (XRCC1: Arg194Trp (OR=1.21, 95% CI: 0.78-1.88); Arg399Gln (OR=1.20, 95% CI: 0.80-1.79); XRCC3: Thr241Met (OR=1.04, 95% CI: 0.76-1.41).

Conclusions: These results suggest that independently these polymorphisms of XRCC1 and XRCC3 genes do not contribute significantly to the genetic susceptibility of breast cancer.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Breast Neoplasms / genetics*
  • Case-Control Studies
  • Cohort Studies
  • DNA Repair
  • DNA-Binding Proteins / genetics*
  • Female
  • Genotype
  • Humans
  • Iowa
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Genetic / genetics*
  • Polymorphism, Restriction Fragment Length
  • Prospective Studies
  • Risk Factors
  • Surveys and Questionnaires
  • X-ray Repair Cross Complementing Protein 1


  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • X-ray repair cross complementing protein 3
  • XRCC1 protein, human