Genetic characterization of the resorcinol catabolic pathway in Corynebacterium glutamicum

Appl Environ Microbiol. 2006 Nov;72(11):7238-45. doi: 10.1128/AEM.01494-06. Epub 2006 Sep 8.


Corynebacterium glutamicum grew on resorcinol as a sole source of carbon and energy. By genome-wide data mining, two gene clusters, designated NCgl1110-NCgl1113 and NCgl2950-NCgl2953, were proposed to encode putative proteins involved in resorcinol catabolism. Deletion of the NCgl2950-NCgl2953 gene cluster did not result in any observable phenotype changes. Disruption and complementation of each gene at NCgl1110-NCgl1113, NCgl2951, and NCgl2952 indicated that these genes were involved in resorcinol degradation. Expression of NCgl1112, NCgl1113, and NCgl2951 in Escherichia coli revealed that NCgl1113 and NCgl2951 both coded for hydroxyquinol 1,2-dioxygenases and NCgl1112 coded for maleylacetate reductases. NCgl1111 encoded a putative monooxygenase, but this putative hydroxylase was very different from previously functionally identified hydroxylases. Cloning and expression of NCgl1111 in E. coli revealed that NCgl1111 encoded a resorcinol hydroxylase that needs NADPH as a cofactor. E. coli cells containing Ncgl1111 and Ncgl1113 sequentially converted resorcinol into maleylacetate. NCgl1110 and NCgl2950 both encoded putative TetR family repressors, but only NCgl1110 was transcribed and functional. NCgl2953 encoded a putative transporter, but disruption of this gene did not affect resorcinol degradation by C. glutamicum. The function of NCgl2953 remains unclear.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism*
  • Corynebacterium glutamicum / enzymology
  • Corynebacterium glutamicum / genetics
  • Corynebacterium glutamicum / growth & development
  • Corynebacterium glutamicum / metabolism*
  • Dioxygenases / genetics
  • Dioxygenases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Bacterial*
  • Genetic Complementation Test
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism
  • Multigene Family
  • Oxidoreductases Acting on CH-CH Group Donors / genetics
  • Oxidoreductases Acting on CH-CH Group Donors / metabolism
  • Resorcinols / metabolism*


  • Bacterial Proteins
  • Resorcinols
  • Mixed Function Oxygenases
  • Dioxygenases
  • hydroxyquinol 1,2-dioxygenase
  • resorcinol hydroxylase
  • Oxidoreductases Acting on CH-CH Group Donors
  • maleylacetate reductase
  • resorcinol