Phylogenetic analysis of human parvovirus B19, indicating two subgroups of genotype 1 in Vietnamese patients

J Gen Virol. 2006 Oct;87(Pt 10):2941-2949. doi: 10.1099/vir.0.82037-0.


Recently, three distinct genotypes (1, 2 and 3) of human parvovirus B19 (B19) have been identified. However, the characteristics and distribution of B19 genotypes in Vietnam have not been investigated. Phylogenetic analysis using 49 subgenomic NS1/VP1u regions and two coding NS1-VP1/VP2 regions has been applied to investigate the prevalence of B19 genotypes in Vietnamese patients co-infected with Hepatitis B virus. Genetic analysis of the subgenomic NS1/VP1u region of B19 revealed that two genotypes of B19 were identified in these populations, with predominance of genotype 1 (47/49, 96 %) followed by genotype 2 (2/49, 4 %), but not genotype 3. Further, phylogenetic analysis of subgenomic B19 genomes revealed two major subgroups within genotype 1 (B19-1A and B19-1B) with an estimated nucleotide difference of >5 % between each subgroup, forming different branches. The mean percentage of amino acid variation between subgroup B19-1A and B19-1B was >2 % of the NS1, VP1 and VP2 proteins. Our results indicated that two of the three known genotypes of B19 were present in Vietnamese patients, with genotype 1 predominating, and that this genotype can be classified into at least two subgroups, B19-1A and B19-1B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Parvoviridae Infections / epidemiology
  • Parvoviridae Infections / virology*
  • Parvovirus B19, Human / classification*
  • Parvovirus B19, Human / genetics*
  • Phylogeny*
  • Vietnam / epidemiology

Associated data

  • GENBANK/DQ357064
  • GENBANK/DQ357065