Silica-guanidinium thiocyanate-based nucleic acid isolation protocol does not improve sensitivity of two commercial tests for detection of Mycobacterium tuberculosis in respiratory samples

Eur J Clin Microbiol Infect Dis. 2006 Oct;25(10):673-5. doi: 10.1007/s10096-006-0194-z.

Authors

H R van Doorn¹, E S Bruijnesteijn van Coppenraet, B Duim, C M J E Vandenbroucke-Grauls, J F Weel, J Dankert, E J Kuijper, M D de Jong

Affiliation

¹ Department of Medical Microbiology, Room L1-245, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100DE, Amsterdam, The Netherlands. h.r.vandoorn@amc.uva.nl

PMID: 16964511
DOI: 10.1007/s10096-006-0194-z

No abstract available

MeSH terms

Bronchoalveolar Lavage Fluid / microbiology
Guanidines / chemistry*
Humans
Mycobacterium tuberculosis / isolation & purification*
Nucleic Acids / isolation & purification*
Sensitivity and Specificity
Silicon Dioxide / chemistry*
Sputum / microbiology
Thiocyanates / chemistry*
Tuberculosis / microbiology*

Substances

Guanidines
Nucleic Acids
Thiocyanates
guanidine thiocyanate
Silicon Dioxide