Abstract
Quantum dots (QDs) are often cell-impermeable and require transporters to facilitate crossing over cell membranes. Here we present a simple and versatile method that utilizes enzymes, matrix metalloprotease 2 (MMP-2) and MMP-7, to modulate the cellular uptake of QDs. QD-peptide conjugates could be efficiently taken up into cells after the MMP treatment. This enzyme-modulated cellular uptake of QDs may be applied to other nanoparticles for biological imaging and selective drug delivery into tumor cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Coated Materials, Biocompatible / chemistry
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Coated Materials, Biocompatible / pharmacokinetics*
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Fibrosarcoma / metabolism*
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Fibrosarcoma / pathology*
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Humans
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Materials Testing
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Matrix Metalloproteinase 2 / chemistry
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Matrix Metalloproteinase 2 / pharmacokinetics*
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Matrix Metalloproteinase 7 / chemistry
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Matrix Metalloproteinase 7 / pharmacokinetics*
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Molecular Probe Techniques*
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Quantum Dots*
Substances
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Coated Materials, Biocompatible
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Matrix Metalloproteinase 7
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Matrix Metalloproteinase 2