Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell

Mol Cell Biol. 1990 Sep;10(9):4846-53. doi: 10.1128/mcb.10.9.4846-4853.1990.

Abstract

32DC13(G) is an interleukin-3-dependent murine hematopoietic precursor cell line which differentiates into neutrophilic granulocytes upon exposure to granulocyte colony-stimulating factor (G-CSF) but ceases to proliferate and dies when exposed to granulocyte-macrophage (GM)-CSF. Surface receptors for GM-CSF are undetectable on 32DC13(G) cells but can be induced by priming the cells with G-CSF. Exposure of the G-CSF-primed cells to GM-CSF then results in the generation of monocytes as well as granulocytes. The acquired competence to respond to GM-CSF remains irreversibly encoded in the primed cells, although the GM-CSF receptor can be down regulated by interleukin-3. This phenomenon suggests a mechanism by which hematopoietic precursors may obtain additional receptors, thereby increasing their differentiative potential.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects*
  • Cell Division / drug effects
  • Cell Line
  • Colony-Stimulating Factors / metabolism*
  • Colony-Stimulating Factors / pharmacology*
  • Granulocyte Colony-Stimulating Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Growth Substances / metabolism
  • Growth Substances / pharmacology
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / physiology
  • Interleukin-3 / pharmacology
  • Kinetics
  • Mice
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / drug effects
  • Receptors, Colony-Stimulating Factor
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology

Substances

  • Colony-Stimulating Factors
  • Growth Substances
  • Interleukin-3
  • Receptors, Cell Surface
  • Receptors, Colony-Stimulating Factor
  • Recombinant Proteins
  • Granulocyte Colony-Stimulating Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor