Cross-reactive protection against influenza A virus infections by an NS1-specific CTL clone

Virology. 1990 Sep;178(1):174-9. doi: 10.1016/0042-6822(90)90391-4.


An influenza A subtype cross-reactive CTL clone (A-11) was established following stimulation of A/PR/8 virus-immune spleen cells of Balb/C (H-2d) mice. This T cell clone lysed target cells infected with influenza viruses of the H1, H2, or H3 subtypes, and recognizes a conserved epitope on the NS1 protein. The clone is restricted by the H-2Ld allele. Adoptive transfer of A-11 significantly reduced virus titers in the lungs of mice infected with influenza A viruses of the H1, H2, or H3 subtypes. These results suggest that the conserved epitope on NS1 which is recognized by A-11 may be a useful component to consider for inclusion in experimental cross-reactive influenza vaccines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Capsid / genetics
  • Capsid / immunology*
  • Cell Line
  • Clone Cells
  • Cross Reactions
  • Epitopes / genetics
  • Epitopes / immunology
  • Influenza A virus / genetics
  • Influenza A virus / immunology
  • Lung / immunology
  • Lung / microbiology
  • Major Histocompatibility Complex / genetics
  • Major Histocompatibility Complex / immunology
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections / genetics
  • Orthomyxoviridae Infections / immunology*
  • Viral Core Proteins / genetics
  • Viral Core Proteins / immunology*
  • Viral Nonstructural Proteins


  • Epitopes
  • Viral Core Proteins
  • Viral Nonstructural Proteins