Nitrosyl-hemoglobin formation in the blood of animals exposed to nitric oxide

Arch Environ Health. 1975 Sep;30(9):453-6. doi: 10.1080/00039896.1975.10666749.


Animals were exposed to nitric oxide (NO) in order to study its biological effect. First a method was developed to exclude nitrogen dioxide formed during exposure of NO. Nitrosyl-hemoglobin (NOHb) in the blood of mice, rats, and rabbits exposed to NO was determined by electron spin resonance spectrometry at -100C. The content of NOHb in the blood of the mouse reached the maximum (0.13% of the total hemoglobin) 20 minutes after the exposure was begun and thereafter a state of equilibrium was maintained. The NOHv level decreased to one half in several minutes after the mouse was removed to a filtered air environment. Dose-response relationship was observed between gas concentrations and the maximum NOHb level.

MeSH terms

  • Animals
  • Atmosphere Exposure Chambers
  • Dose-Response Relationship, Drug
  • Electron Spin Resonance Spectroscopy
  • Environmental Exposure
  • Female
  • Hemoglobins / analysis*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Mice, Inbred Strains
  • Nitric Oxide / toxicity*
  • Nitroso Compounds / blood
  • Rabbits
  • Rats


  • Hemoglobins
  • Nitroso Compounds
  • Nitric Oxide