Application of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 high volume clinical chemistry analyzer

Clin Biochem. 2006 Oct;39(10):1041-3. doi: 10.1016/j.clinbiochem.2006.06.011. Epub 2006 Jul 21.

Abstract

Objectives: Evaluation of the performance of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 analyzer.

Design and methods: Imprecision studies were performed and patient samples were assayed by EMIT assay and by LC-MS/MS.

Results: Limit of quantification was established at 2.8 microg/L. A positive bias of 17.5% between results measured on EMIT and on LC-MS/MS was detected.

Conclusions: EMIT 2000 Tacrolimus assay is suitable for automated analyses of Tacrolimus on the Architect c8000.

MeSH terms

  • Chromatography, Liquid
  • Clinical Chemistry Tests / instrumentation*
  • Humans
  • Immunosuppressive Agents / blood*
  • Mass Spectrometry
  • Reference Standards
  • Reproducibility of Results
  • Tacrolimus / blood*

Substances

  • Immunosuppressive Agents
  • Tacrolimus